Abstract

Isolation and Diversity Analysis of Soil Metagenome

Author(s): Tanvi,Vivek Srivastava, Zeenat Wadhwa, Raj Rani, Kanchan Makkar and Sumit Jangra

Soil biology holds an important role for methodological studies. Among different niches, the highestlevel of prokaryotic diversity resides in the soil, and extraction of DNA from soil using metagenomic approaches can improve our knowledge about these communities. The analyses of soil biodiversity and utility undertake that the extracted DNA denotes the complete microbial community of that niche,but further elucidations are limited due to the amount of DNA recovered from the sample. To improve metagenomic approaches, the present study aimed at determining the effect of different concentrations of SDS and depth of soil sample from the surface on microbial diversity in different areas. Maximum amount of DNA (1.53 μg/g) was obtained by using CTAB along with 2% SDS. Soil samples taken from a depth of 16 cm resulted in maximum DNA isolation which was further enhanced due to vortexing procedure (402.46,509.6 and 2289.8 in case of NS 16cm, waste soil 16cm and Durgapura soil 16cm respectively). The quality of DNA increased with the ratio A260/280 varying from 1.30 to 1.61 for RNase based and 1.69 to 1.81 for column based purification.

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