Abstract

Simple and Effective Method for Development of Longer Shelf Life Liquid Formulation with Azospirillum brasilense strain Asp-7

Author(s): Mounika N., Muralidhara Rao D., Uma A., Sandhya V.and Ali SkZ

Liquid formulation is an upcoming technology to overcome the limitations associated with carrier based formulation. In our study a total of nine Azospirillum spp. were isolated, screened for plant growth promoting traits (PGP) like indole acetic acid (IAA), ammonia, phosphatesolubilisation and siderophore production. The se-lected strain Asp-7 was showed tolerance to droughtand temperature stress.Biochemical and molecular characteri-zation of the strain Asp-7 was done and identified as Azospirillum brasilense by 16S rDNA sequence analysis and the gene sequence was submitted to Genbank under the accession number KT374217. The Azospirillum brasilensestrain Asp-7 was further screened with different polymeric additives to support growth and shelflife of liquid formulation. Among the additives tested PVP at 4% concentration showed thehighest cell population.Liquid formulation ofAzospirillum brasilense strain Asp-7 formulated with PVP was evaluated for its shelf life for16 months. The results of the present study confirmed that addition of PVP has enhanced theshelf life of liquid formulation of Azospirillum brasilense strain Asp-7.

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